Effect of a direct-fed microbial (Eubios 1090) in the presence of antibiotics (Carbadox or CTC-Denagard) on post-weaning pig growth performance and immune response

A study was conducted to determine the effects of a probiotic (Eubios 1090), in the presence of two different antibiotics, on performance in nursery pigs. A total of 216 pigs were weaned at an average of 21 d, blocked by initial body weight (BW = 6.79 kg), and distributed into 32 pens of 6 to 7 pigs per pen in an offsite nursery facility. Pens were randomly assigned to 1 of 4 dietary treatments (8 pens per treatment) that were fed throughout post-weaning phase 1 (day (D) 0 to 10), phase 2 (D 10 to 20), and phase 3 (D 20 to 34). Dietary treatments were: 1) Carbadox without Eubios 1090; 2) Chlortetracycline + Tiamulin (CTC-Denagard) without Eubios 1090; 3) Carbadox + Eubios 1090; and 4) CTC-Denagard + Eubios 1090. There was no interaction observed between the two antibiotics and addition of the probiotic. There was a tendency for greater gain to feed ratio (G:F) in phase 2 when nursery pigs received Carbadox compared to CTC-Denagard (P = 0.08), and a tendency for greater average daily feed intake (ADFI) in the overall nursery period when pigs were fed CTCDenagard compared to Carbadox (P = 0.10). Pigs that received the non-Eubios 1090 diets had greater average daily gain (ADG), G:F, and body weight (BW) during phase 2 compared to pigs that received diets containing Eubios 1090 (P = 0.05). In phase 3, pigs receiving the Eubios 1090 diet had increased ADG and G:F (P = 0.05). Between the Carbadox diet and the CTC-Denagard diet, the diet containing CTC-Denagard increased ADFI throughout the 3 phases. In summary, probiotic supplementation demonstrated negative effects in phase 2 and positive effects to growth performance in nursery pigs during the latter part of early post-weaning (phase 3)

FACTORES ASOCIADOS A LAS COMPLICACIONES DESPUÉS DEL PARTO EN MUJERES DE 12 A 49 AÑOS EN PERÚ, SEGÚN LA ENCUESTA DEMOGRÁFICA Y SALUD FAMILIAR, 2019-2020

Introducción: Las complicaciones obstétricas son causa de morbimortalidad materna a nivel mundial, estas se pueden presentar durante el embarazo, el parto o puerperio. El puerperio es la etapa con mayor porcentaje de complicaciones, a pesar de que nuestro país logro disminuir la mortalidad materna en un 73% sigue siendo una amenaza para las mujeres y constituye un problema de salud pública importante. Objetivo: Determinar los factores asociados a las complicaciones después del parto en mujeres de 12 a 49 años en Perú, según la encuesta demográfica y salud familiar, 2019- 2020. Métodos: Estudio de tipo cuantitativo, observacional, retrospectivo, transversal y analítico, basado en ENDES 2019-2020. Resultados: Se obtuvo una prevalencia de complicaciones después del parto de 37.7% En el análisis multivariado, se encontró una asociación significativa de los factores sociodemográficos: edad de 20 a 35 años (RPa: 1.12, IC95%: 1.07 a 1.18). Las mujeres con complicaciones durante el parto tienen 2.7 más veces la prevalencia de complicaciones después del parto. El grado de instrucción primaria (RPa: 0.90, IC95%: 0.84 a 0.96), secundaria (RPa: 0.94, IC95%: 0.89 a 0.98) y tener estado civil soltera (RPa 0,85, IC95%: 0,76 a 0,94) disminuyen la probabilidad de tener complicaciones después delparto. Noseencontróunaasociaciónsignificativaconetnia,estadocivil,tipodelugar de residencia, región, ingresos económicos, chequeos postnatales, parto por cesárea, lugar de parto y violencia física y emocional (p>0.05). Conclusiones: La prevalencia de las complicaciones después del parto es de 37.7%. Los factores asociados a las complicaciones después del parto son: la edad, grado de instrucción, estado civil y complicaciones durante el parto

Prokaryotic Community Structure and Metabolisms in Shallow Subsurface of Atacama Desert Playas and Alluvial Fans After Heavy Rains: Repairing and Preparing for Next Dry Period

The Atacama Desert, the oldest and driest desert on Earth, displays significant rains only once per decade. To investigate how microbial communities take advantage of these sporadic wet events, we carried out a geomicrobiological study a few days after a heavy rain event in 2015. Different physicochemical and microbial community analyses were conducted on samples collected from playas and an alluvial fan from surface, 10, 20, 50, and 80 cm depth. Gravimetric moisture content peaks were measured in 10 and 20 cm depth samples (from 1.65 to 4.1% w/w maximum values) while, in general, main anions such as chloride, nitrate, and sulfate concentrations increased with depth, with maximum values of 13-1,125; 168-10,109; and 9,904-30,952 ppm, respectively. Small organic anions such as formate and acetate had maximum concentrations from 2.61 to 3.44 ppm and 6.73 to 28.75 ppm, respectively. Microbial diversity inferred from DNA analysis showed Actinobacteria and Alphaproteobacteria as the most abundant and widespread bacterial taxa among the samples, followed by Chloroflexi and Firmicutes at specific sites. Archaea were mainly dominated by Nitrososphaerales, Methanobacteria, with the detection of other groups such as Halobacteria. Metaproteomics showed a high and even distribution of proteins involved in primary metabolic processes such as energy production and biosynthetic pathways, and a limited but remarkable presence of proteins related to resistance to environmental stressors such as radiation, oxidation, or desiccation. The results indicated that extra humidity in the system allows the microbial community to repair, and prepare for the upcoming hyperarid period. Additionally, it supplies biomarkers to the medium whose preservation potential could be high under strong desiccation conditions and relevant for planetary exploration.Spanish Ministerio de Economia y Competitividad/Fondo Europeo de Desarrollo Regional Grants [ESP2014-58494-R, ESP2015-69540-R, ESP2017-89053-C2-2-P]; NASA ASTEP "Life in the Atacama (LITA)" project [NNX11AJ87G]; NASA Astrobiology Institute Colaborative Agreement 7 (CAN-7) project [NNX15BB01A]; Comunidad de Madrid/European Union YEI program; Spanish Ministry of Economy and Competitivity (MINECO); "Maria de Maeztu" program [MDM-2017-0737]Open access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]

Molecular characterisation of protist parasites in human-habituated mountain gorillas (Gorilla beringei beringei), humans and livestock, from Bwindi impenetrable National Park, Uganda

Over 60 % of human emerging infectious diseases are zoonotic, and there is growing evidence of the zooanthroponotic transmission of diseases from humans to livestock and wildlife species, with major implications for public health, economics, and conservation. Zooanthroponoses are of relevance to critically endangered species; amongst these is the mountain gorilla (Gorilla beringei beringei) of Uganda. Here, we assess the occurrence of Cryptosporidium, Cyclospora, Giardia, and Entamoeba infecting mountain gorillas in the Bwindi Impenetrable National Park (BINP), Uganda, using molecular methods. We also assess the occurrence of these parasites in humans and livestock species living in overlapping/adjacent geographical regions

Fine-mapping of the HNF1B multicancer locus identifies candidate variants that mediate endometrial cancer risk.

Common variants in the hepatocyte nuclear factor 1 homeobox B (HNF1B) gene are associated with the risk of Type II diabetes and multiple cancers. Evidence to date indicates that cancer risk may be mediated via genetic or epigenetic effects on HNF1B gene expression. We previously found single-nucleotide polymorphisms (SNPs) at the HNF1B locus to be associated with endometrial cancer, and now report extensive fine-mapping and in silico and laboratory analyses of this locus. Analysis of 1184 genotyped and imputed SNPs in 6608 Caucasian cases and 37 925 controls, and 895 Asian cases and 1968 controls, revealed the best signal of association for SNP rs11263763 (P = 8.4 × 10(-14), odds ratio = 0.86, 95% confidence interval = 0.82-0.89), located within HNF1B intron 1. Haplotype analysis and conditional analyses provide no evidence of further independent endometrial cancer risk variants at this locus. SNP rs11263763 genotype was associated with HNF1B mRNA expression but not with HNF1B methylation in endometrial tumor samples from The Cancer Genome Atlas. Genetic analyses prioritized rs11263763 and four other SNPs in high-to-moderate linkage disequilibrium as the most likely causal SNPs. Three of these SNPs map to the extended HNF1B promoter based on chromatin marks extending from the minimal promoter region. Reporter assays demonstrated that this extended region reduces activity in combination with the minimal HNF1B promoter, and that the minor alleles of rs11263763 or rs8064454 are associated with decreased HNF1B promoter activity. Our findings provide evidence for a single signal associated with endometrial cancer risk at the HNF1B locus, and that risk is likely mediated via altered HNF1B gene expression